Data file: sample.dat Number of sequences 47 Sample data file. If you use the following sample config: 20 27 and do 1000 permutations the results should be those given in the last line of Table 4 in Hudson, et al MBE 9:138-151. OTUs 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 0 7 7 5 10 3 7 8 9 6 7 11 12 8 4 5 5 9 5 7 5 10 7 7 4 11 8 7 4 4 8 10 9 10 6 4 6 5 12 10 10 8 10 9 8 4 5 1 12 10 9 6 6 5 10 7 8 12 13 7 7 6 8 10 8 8 8 5 10 12 5 8 7 8 5 5 7 9 12 9 7 7 5 8 9 5 5 5 9 10 9 7 6 2 6 9 8 8 9 10 5 10 8 9 9 7 8 8 6 8 6 4 9 8 6 7 8 9 10 7 7 9 11 6 11 5 7 7 6 9 9 9 11 11 8 5 7 8 3 7 8 8 7 12 5 8 8 9 7 5 6 8 8 8 4 4 9 8 6 5 8 9 12 5 7 9 11 8 11 3 5 7 6 9 9 9 9 11 8 7 7 6 4 11 7 6 11 8 9 5 6 10 8 9 9 5 9 5 7 8 9 5 8 7 8 11 8 8 10 10 11 8 4 8 8 7 4 8 8 8 12 9 8 10 9 5 8 9 10 7 8 12 13 5 5 6 6 10 6 8 6 7 8 8 5 12 9 8 5 5 9 11 10 11 7 5 7 6 13 7 7 9 9 10 9 7 8 6 3 8 5 8 10 11 7 7 6 4 6 4 4 4 5 6 8 5 4 5 6 5 3 7 9 8 7 5 7 5 2 7 5 5 5 7 6 5 5 6 7 9 4 7 9 10 6 6 5 7 7 7 5 5 4 7 9 4 5 6 9 4 4 6 8 9 8 4 6 4 5 6 4 4 4 8 7 6 6 5 8 7 10 12 13 11 9 8 8 8 8 12 8 9 10 12 9 10 9 10 9 5 3 5 8 9 9 9 7 8 9 9 9 7 9 8 7 5 6 9 7 7 8 4 4 3 7 5 7 5 3 4 7 7 2 5 8 11 2 4 4 6 5 6 4 4 2 5 6 4 4 6 6 7 4 4 3 10 12 13 9 5 6 6 10 6 8 8 9 10 10 7 10 7 10 7 5 7 9 12 9 5 5 7 6 11 9 9 7 7 12 9 7 6 11 1 11 9 8 10 6 10 6 8 9 8 6 9 6 9 12 9 11 9 9 6 7 7 9 9 10 5 9 9 11 13 10 7 11 10 12 12 10 9 11 7 11 7 9 10 9 7 10 7 10 13 10 12 10 10 7 8 8 10 10 11 6 10 10 12 14 11 8 12 11 13 4 5 9 9 9 7 5 4 9 9 4 9 10 13 4 6 8 8 9 10 6 4 6 7 10 4 4 8 8 9 8 8 7 14 3 5 9 5 5 5 8 7 7 4 9 6 9 4 4 6 6 9 8 4 0 6 5 10 8 8 6 8 9 8 6 5 15 4 8 4 6 4 7 8 8 3 6 7 10 3 3 5 7 6 5 5 3 5 6 9 7 7 5 7 8 5 5 4 16 8 0 6 6 9 8 8 7 6 5 6 7 3 7 9 8 5 5 5 7 2 11 9 9 7 7 10 5 5 6 17 8 8 4 7 10 4 7 6 11 12 7 5 7 7 6 5 5 9 5 6 3 7 7 9 9 8 3 5 6 18 6 6 9 8 8 7 6 5 6 7 3 7 9 8 5 5 5 7 2 11 9 9 7 7 10 5 5 6 19 4 7 6 6 5 4 5 8 5 7 9 11 8 9 3 5 7 4 7 7 7 7 9 6 7 9 8 20 7 6 4 3 6 9 10 3 3 7 9 4 9 3 5 5 4 7 7 7 7 9 4 3 5 6 21 9 11 6 5 8 11 6 6 6 8 9 8 6 8 4 7 6 0 0 6 6 9 6 6 5 22 8 7 10 7 8 7 7 9 11 8 13 7 7 9 6 11 9 9 9 13 8 9 9 10 23 7 10 11 12 7 7 11 11 6 9 5 7 9 6 7 11 11 11 13 6 7 9 10 24 7 8 9 0 4 6 8 7 8 4 4 2 5 8 6 6 6 8 7 6 6 5 25 7 10 7 7 7 9 6 5 5 9 5 6 5 5 5 7 7 8 3 7 6 26 3 8 6 6 8 11 8 6 6 8 5 10 8 8 6 8 11 10 8 7 27 9 7 9 11 12 11 9 9 9 6 13 11 11 9 11 12 11 9 10 28 4 6 8 7 8 4 4 2 5 8 6 6 6 8 7 6 6 5 29 4 6 7 6 4 4 4 3 8 6 6 4 6 7 4 2 3 30 2 7 6 6 6 4 7 6 6 6 4 6 9 6 4 3 31 9 6 8 6 6 9 6 8 8 6 8 11 8 6 5 32 9 7 9 7 8 9 9 9 11 11 6 3 7 8 33 8 8 6 7 6 8 8 8 6 13 6 6 5 34 4 4 3 6 6 6 6 8 7 4 6 5 35 6 5 10 8 8 6 8 9 8 6 5 36 5 6 4 4 6 6 9 4 4 3 37 9 7 7 7 7 8 5 5 6 38 6 6 6 10 7 6 8 7 39 0 6 6 9 6 6 5 40 6 6 9 6 6 5 41 8 7 8 6 5 42 13 8 6 5 43 7 9 10 44 4 5 45 1 Sample configuration: 12 8 27 Number of permutations: 1000 weighting constant: 0 Observed values of statistics: Kst: -0.003785 , Ks: 7.196421 Kt: 7.169288 ( p-value: 0.619000) 1 2: Kst: -0.011015 , Ks: 7.603896 Kt: 7.521053 ( p-value: 0.682000) 1 3: Kst: -0.006720 , Ks: 7.244038 Kt: 7.195682 ( p-value: 0.764000) 2 3: Kst: 0.005380 , Ks: 6.910518 Kt: 6.947899 ( p-value: 0.278000) Sample configuration: 12 8 27 Number of permutations: 1000 weighting constant: 2 Observed values of statistics: K*st: 0.002736 , K*s: 2.045753 K*t: 2.051367 ( p-value: 0.305000) 1 2: K*st: -0.002582 , K*s: 2.101032 K*t: 2.095620 ( p-value: 0.573000) 1 3: K*st: -0.000116 , K*s: 2.055481 K*t: 2.055243 ( p-value: 0.473000) 2 3: K*st: 0.005954 , K*s: 2.006239 K*t: 2.018256 ( p-value: 0.150000) Sample configuration: 12 8 27 Number of permutations: 1000 weighting constant: 2 Observed values of statistics: Hst: 0.006662 , Hs: 0.987825 Ht: 0.994450 ( p-value: 0.043000) 1 2: Hst: 0.008173 , Hs: 0.986607 Ht: 0.994737 ( p-value: 0.170000) 1 3: Hst: 0.002757 , Hs: 0.991860 Ht: 0.994602 ( p-value: 0.221000) 2 3: Hst: 0.006080 , Hs: 0.983897 Ht: 0.989916 ( p-value: 0.085000) Sample configuration: 12 8 27 Number of permutations: 1000 weighting constant: 2 Observed values of statistics: Zst: -0.007284 , Zs: 543.933341 Zt: 540.000000 ( p-value: 0.639000) 1 2: Zst: -0.037794 , Zs: 606.369251 Zt: 584.286842 ( p-value: 0.895000) 1 3: Zst: -0.005108 , Zs: 547.096404 Zt: 544.315789 ( p-value: 0.594000) 2 3: Zst: 0.006173 , Zs: 508.137156 Zt: 511.293277 ( p-value: 0.334000) Sample configuration: 12 8 27 Number of permutations: 1000 weighting constant: 2 Observed values of statistics: Z*st: 0.001397 , Z*s: 5.991609 Z*t: 5.999990 ( p-value: 0.397000) 1 2: Z*st: -0.002166 , Z*s: 6.135791 Z*t: 6.122532 ( p-value: 0.569000) 1 3: Z*st: -0.001091 , Z*s: 6.018354 Z*t: 6.011794 ( p-value: 0.571000) 2 3: Z*st: 0.004581 , Z*s: 5.886996 Z*t: 5.914086 ( p-value: 0.195000) Sample configuration: 12 8 27 Test of Roff and Bentzen MBE 6: 539-45 Number of permutations: 1000 Observed values of statistics: Number of alleles: 42. Ht: 0.994450 Chi: 90.192130 ( p-value: 0.110000) 1 2: Number of alleles: 19. Ht: 0.994737 Chi: 20.000000 ( p-value: 0.509000) 1 3: Number of alleles: 36. Ht: 0.994602 Chi: 39.000000 ( p-value: 0.199000) 2 3: Number of alleles: 30. Ht: 0.989916 Chi: 32.164352 ( p-value: 0.300000) Sample configuration: 12 8 27 Number of permutations: 1000 Global test: Snn: 0.556442 ( p-value: 0.031000) Pairwise tests of samples: 1 2: Snn: 0.566667 ( p-value: 0.335000) 1 3: Snn: 0.686610 ( p-value: 0.070000) 2 3: Snn: 0.693129 ( p-value: 0.195000)