First International Norrbyskar Conference July 3-5, 1999
Cell Physiology at the University of Chicago
Research Interests
Roe, M. W., Fiekers,
J. F., Philipson, L. H., and Bindokas, V. P. (2006). Visualizing calcium
signaling in cells by digitized wide-field and confocal fluorescent microscopy.
Methods Mol Biol 319, 37-66.
Jacobson, D. A.,
Cho, J., Landa, L. R., Tamarina, N. A., Roe, M. W., Buxbaum, J. D., and
Philipson, L. H. (2006). The Downstream Regulatory Element Antagonistic
Modulator Regulates Islet Prodynorphin Expression. Am J Physiol Endocrinol
Metab.
Gimi, B., Leoni, L.,
Oberholzer, J., Braun, M., Avila, J., Wang, Y., Desai, T., Philipson, L. H.,
Magin, R. L., and Roman, B. B. (2006). Functional MR microimaging of pancreatic
beta-cell activation. Cell Transplant 15, 195-203.
Fridlyand, L. E.,
and Philipson, L. H. (2006). Cold climate genes and the prevalence of type 2
diabetes mellitus. Med Hypotheses.
Fridlyand, L. E.,
and Philipson, L. H. (2006). Reactive species and early manifestation of
insulin resistance in type 2 diabetes. Diabetes Obes Metab 8, 136-145.
Fridlyand, L. E.,
and Philipson, L. H. (2006). Oxidative reactive species in cell injury:
mechanisms in diabetes mellitus and therapeutic approaches. Ann N Y Acad Sci
1066, 136-151.
Fridlyand, L. E.,
and Philipson, L. H. (2006). Reactive
Species, Cellular Repair and Risk Factors in the Onset of Type 2 Diabetes
Mellitus: Review and Hypothesis. Curr Diab
Rev 2, in press.
Chong, A. S., Shen,
J., Tao, J., Yin, D., Kuznetsov, A., Hara, M., and Philipson, L. H. (2006).
Reversal of diabetes in NOD mice without spleen cell-derived beta-cell
regeneration Science 311, 1774-1775.
Tamarina, N. A.,
Kuznetsov, A., Rhodes, C. J., Bindokas, V. P., and Philipson, L. H. (2005).
Inositol (1,4,5)-trisphosphate dynamics and intracellular calcium oscillations
in pancreatic beta-cells. Diabetes 54, 3073-3081.
Tamarina, N. A.,
Kuznetsov, A., Fridlyand, L. E., and Philipson, L. H. (2005). Delayed-rectifier
(KV2.1) regulation of pancreatic beta-cell calcium responses to glucose:
inhibitor specificity and modeling. Am J Physiol Endocrinol Metab 289,
E578-585.
Kuznetsov, A.,
Bindokas, V. P., Marks, J. D., and Philipson, L. H. (2005). FRET-based voltage
probes for confocal imaging: membrane potential oscillations throughout
pancreatic islets. Am J Physiol Cell Physiol 289, C224-229.
Fridlyand, L. E.,
Ma, L., and Philipson, L. H. (2005). Adenine nucleotide regulation in
pancreatic beta-cells: modeling of ATP/ADP-Ca2+ interactions. Am J Physiol
Endocrinol Metab 289, E839-848.
Philipson, L. H.,
and Roe, M. W. (2004). Imaging Metabolic and Signaling Targets in the
Pancreatic B-cell. Curr Med Chem: Immunol, Endo, Metab Agents 4, 333-337.
Ma, L., Bindokas, V.
P., Kuznetsov, A., Rhodes, C., Hays, L., Edwardson, J. M., Ueda, K., Steiner,
D. F., and Philipson, L. H. (2004). Direct imaging shows that insulin granule
exocytosis occurs by complete vesicle fusion. Proc Natl Acad Sci U S A 101,
9266-9271.
Houamed, K., Fu, J.,
Roe, M. W., and Philipson, L. H. (2004). Electrophysiology of the Pancreatic
beta cell, In Diabetes Mellitus: A Fundamental and clinical text, D. LeRoith,
S. I. Taylor, and J. O. Olefsky, eds. (Lippincott, Williams and Wilkins).
Fridlyand, L. E.,
and Philipson, L. H. (2004). Does the glucose-dependent insulin secretion mechanism
itself cause oxidative stress in pancreatic beta-cells? Diabetes 53, 1942-1948.
Yaekura, K., Julyan,
R., Wicksteed, B. L., Hays, L. B., Alarcon, C., Sommers, S., Poitout, V.,
Baskin, D. G., Wang, Y., Philipson, L. H., and Rhodes, C. J. (2003). Insulin
secretory deficiency and glucose intolerance in Rab3A null mice. J Biol Chem.
Ueda, K., Lipkind,
G. M., Zhou, A., Zhu, X., Kuznetsov, A., Philipson, L., Gardner, P., Zhang, C.,
and Steiner, D. F. (2003). Mutational analysis of predicted interactions between
the catalytic and P domains of prohormone convertase 3 (PC3/PC1). Proc Natl
Acad Sci U S A 100, 5622-5627.
Tamarina, N. A.,
Wang, Y., Mariotto, L., Kuznetsov, A., Bond, C., Adelman, J., and Philipson, L.
H. (2003). Small-conductance calcium-activated K+ channels are expressed in
pancreatic islets and regulate glucose responses. Diabetes 52, 2000-2006.
Fridlyand, L. E.,
Tamarina, N., and Philipson, L. H. (2003). Modeling of Ca2+ flux in pancreatic
beta-cells: role of the plasma membrane and intracellular stores. Am J Physiol
Endocrinol Metab 285, E138-154.
Bindokas, V. P.,
Kuznetsov, A., Sreenan, S., Polonsky, K. S., Roe, M. W., and Philipson, L. H.
(2003). Visualizing superoxide production in normal and diabetic rat islets of
Langerhans. J Biol Chem 278, 9796-9801.
Qian, F., Huang, P.,
Ma, L., Kuznetsov, A., Tamarina, N., and Philipson, L. H. (2002). TRP Genes:
Candidates for Nonselective Cation Channels and Store- Operated Channels in
Insulin-Secreting Cells. Diabetes 51 Suppl 1, S183-S189.
Philipson, L. H.
(2002). beta-Agonists and metabolism. J Allergy Clin Immunol 110, S313-317.
Terracciano, C. M.,
Philipson, K. D., and MacLeod, K. T. (2001). Overexpression of the Na(+)/Ca(2+)
exchanger and inhibition of the sarcoplasmic reticulum Ca(2+)-ATPase in ventricular
myocytes from transgenic mice. Cardiovasc Res 49, 38-47.
Shih, D. Q.,
Screenan, S., Munoz, K. N., Philipson, L., Pontoglio, M., Yaniv, M., Polonsky,
K. S., and Stoffel, M. (2001). Loss of HNF-1alpha Function in Mice Leads to
Abnormal Expression of Genes Involved in Pancreatic Islet Development and
Metabolism. Diabetes 50, 2472-2480.
Qian, F., Huang, P.,
Ma, L., Kuznetsov, A., Tamarina, N., and Philipson, L. H. (2001). TRP Genes:
candidates for non-selective cation channels and
store-operated channels
in insulin secreting cells. Diabetes in press.
Bindokas, V. P.,
Lee, J. P., Li, C., Tamarina, N., A., K., Zhou, Y. P., Wang, Y., Ostrega, D.,
Roe, M. W., Thompson, C. B., et al. (2001). Overexpression of Bcl-xL in
Pancreatic Beta cells Alters Mitochondrial Distribution. submitted.
Zhou, Y. P., Pena,
J. C., Roe, M. W., Mittal, A., Levisetti, M., Baldwin, A. C., Pugh, W.,
Ostrega, D., Ahmed, N., Bindokas, V. P., et al. (2000). Overexpression of
Bcl-x(L) in beta-cells prevents cell death but impairs mitochondrial signal for
insulin secretion. Am J Physiol Endocrinol Metab 278, E340-351.
Wilson, S. M., Toth,
P. T., Oh, S. B., Gillard, S. E., Volsen, S., Ren, D., Philipson, L. H., Lee,
E. C., Fletcher, C. F., Tessarollo, L., et al. (2000). The status of voltage-dependent
calcium channels in alpha 1E knock-out mice. J Neurosci 20, 8566-8571.
Ma, L., Tamarina,
N., Wang, Y., Kuznetsov, A., Patel, N., Kending, C., Hering, B. J., and
Philipson, L. H. (2000). Baculovirus-mediated gene transfer into pancreatic islet
cells. Diabetes 49, 1986-1991.
Ma, L., Tamarina,
N., Wang, Y., Kuznetsov, A., Patel, N., Kending, C., Hering, B. J., and
Philipson, L. H. (2000). Baculovirus mediated gene transfer into pancreatic
islet cells. Diabetes (rapid publication) 49, 1986-1991.
Kuznetsov, A., Wang,
Y., Palmer, M. B., and Philipson, L. H. (2000). Video rate confocal imaging of
intracellular free Ca2+ and mitochondrial membrane potential In mouse
pancreatic islets. Biophys J 78, 2306.
Philipson, L. H.
(1999). Beta-cell ion channels: keys to endodermal excitability. Horm Metab Res
31, 455-461.
Kuznetsov, A.,
Blair, N., Pfohl, J., Dukes, I. D., and Philipson, L. H. (1999).
Dominant-Negative Suppression of Kv2.1 Channels. Biophys J 77.
Zhou, Y. P., Teng,
D., Dralyuk, F., Ostrega, D., Roe, M. W., Philipson, L., and Polonsky, K. S.
(1998). Apoptosis in insulin-secreting cells. Evidence for the role of
intracellular Ca2+ stores and arachidonic acid metabolism. J Clin Invest 101,
1623-1632.
Roe, M. W., Worley,
J. F., 3rd, Qian, F., Tamarina, N., Mittal, A. A., Dralyuk, F., Blair, N. T.,
Mertz, R. J., Philipson, L. H., and Dukes, I. D. (1998). Characterization of a
Ca2+ release-activated nonselective cation current regulating membrane
potential and [Ca2+]i oscillations in transgenically derived beta-cells. J Biol
Chem 273, 10402-10410.
Dukes, I. D.,
Sreenan, S., Roe, M. W., Levisetti, M., Zhou, Y. P., Ostrega, D., Bell, G. I.,
Pontoglio, M., Yaniv, M., Philipson, L., and Polonsky, K. S. (1998). Defective
pancreatic beta-cell glycolytic signaling in hepatocyte nuclear
factor-1alpha-deficient mice. J Biol Chem 273, 24457-24464.
Dukes, I. D., Roe,
M. W., Worley, J. F., III , and
Philipson, L. H. (1997). Glucose-induced alterations in ß Cell cytoplasmic
calcium: coupling of intracellular calcium stores and plasma membrane ion
channels. Curr Opin Endocrinol and Metab 4, 262-271.
Roe, M. W., Worley,
J. F., 3rd, Tokuyama, Y., Philipson, L. H., Sturis, J., Tang, J., Dukes, I. D.,
Bell, G. I., and Polonsky, K. S. (1996). NIDDM is associated with loss of
pancreatic beta-cell L-type Ca2+ channel activity. Am J Physiol 270, E133-140.
Roe, M. W., Worley,
J. F., III , Mittal, A. A., Kuznetsov, A., DasGupta, S., Mertz, R. J.,
Witherspoon, S. M., III, Blair, N., Lancaster, M. E., McIntyre, M. S., et al.
(1996). Expression and function of pancreatic ß-cell delayed rectifier K+
channels: role in stimulus-secretion coupling. J Biol Chem 271, 32241-32246.
Philipson, L. H.,
Roe, M. W., Worley III, J. F., Mittal, A., Kuznetsov, A., Blair, N. T., Ghai,
C., McIntyre, M. S., Lancaster, M. E., and Dukes, I. D. (1996). Absence Of
Glucose-Regulated K(ATP) Current In Pancreatic ß- Cells Of Persistent
Hyperinsulinemia. Biophys J 69, abstract.
Marubio, L. M., Roenfeld,
M., Dasgupta, S., Miller, R. J., and Philipson, L. H. (1996). Isoform
expression of the voltage-dependent calcium channel alpha 1E. Receptors
Channels 4, 243-251.
Lee, T. E.,
Philipson, L. H., and Nelson, D. J. (1996). N-type inactivation in the mammalian
Shaker K+ channel Kv1.4. Journal of Membrane Biology 151, 225-235.
Dukes, I. D., and
Philipson, L. H. (1996). K+ channels: generating excitement in pancreatic
beta-cells. Diabetes 45, 845-853.
Philipson, L. H.,
and Steiner, D. F. (1995). Pas de Deux or more: The sulfonylurea receptor and
K+ channels. Science 268, 372-373.
Philipson, L. H.,
Roe, M. W., Mittal, A. A., Kuznetsov, A., and Dukes, I. D. (1995). Regulation
of Ca2+ oscillations in ßTC3 insulinoma cells. Diabetologia 38(S1), A121.
Philipson, L. H.,
Kuznetsov, A., Toth, P. T., Murphy, J., Szabo, G., Ma, G. H., and Miller, R. J.
(1995). Functional expression of an epitope-tagged G-protein coupled K+ channel
(GIRK1). J Biol Chem 270, 14604-14610.
Philipson, L. H.
(1995). ATP-sensitive K+ channels: paradigm lost, paradigm regained. Science
270, 1159.
Maya, D. J., Foose, J. M., Philipson, L. H., and Tamkun, M. M. (1995). Localization of the
Kv1.5 K+ channel protein in explanted cardiac tissue. J Clin Invest 96, 282-292.
Malayev, A. A.,
Nelson, D. J., and Philipson, L. H. (1995). Mechanism of clofilium block of the
human Kv1.5 delayed rectifier potassium channel. Mol Pharm 47, 198-205.
Ma, G. H., Miller,
R. J., Kuznetsov, A., and Philipson, L. H. (1995). k-Opioid
Receptor Activates an Inwardly Rectifying K+ channel (GIRK1) by a G protein
linked mechanism: co-expression in Xenopus oocytes. Mol Pharm 47, 1035-1040.
Kuznetsov, A., Ma,
G. H., Mao, J., Ren, D. J., Worley, J. F. I., Lancaster, M. E., Dukes, I. D.,
and Philipson, L. H. (1995). Dominant-negative suppression of mammalian delayed
rectifier K+ channels by co-expression oif a truncated and tagged channel.
Biophys J 68, A266.
Yano, H., Philipson,
L. H., Kugler, J. L., Tokuyama, Y., Davis, E. M., LeBeau, M. M., Nelson, D. J.,
Bell, G. I., and Takeda, J. (1994). Alternative Splicing of Human Inwardly
rectifying K+ Channel ROMK1 mRNA. Mol Pharm 45, 854-860.
Williams, M. E.,
Marubio, L. M., Deal, C., Hans, M., Brust, P. F., Philipson, L. H., Miller, R.
J., Jophnson, E. C., Harpold, M. M., and Ellis, S. B. (1994). Structure and
functional characterization of neuronal a1E calcium channel subtypes. J Biol Chem 269, :22347-22357.
Stoffel, M.,
Espinosa, R. I., Powell, K. L., Philipson, L. H., LeBeau, M. M., and Bell, G.
I. (1994). Human G-protein-coupled inwardly rectifying potassium channel
(GIRK1) gene (KCNJ3): localization to chromosome 2 and identification of a
simple tandem repeat polymorphism. Genomics 21, 254-256,.
Roe, M. W.,
Philipson, L. H., Frangakis, C. J., Kuznetsov, A., Mertz, R. J., Lancaster, M.
E., Spencer, B., Worley, J. r., and Dukes, I. D. (1994). Defective
glucose-dependent endoplasmic reticulum Ca2+ sequestration in diabetic mouse
islets of Langerhans. Journal of Biological Chemistry 269, 18279-18282.
Philipson, L. H.,
Rosenberg, M. P., Kuznetsov, A., Lancaster, M. E., Worley, J. F., III, Roe, M.
W., and Dukes, I. D. (1994). Delayed rectifier K+ channel overexpression in
transgenic islets and ß-cells associated with impaired glucose responsiveness.
J Biol Chem 269, 27787-27790.
Lee, T. E.,
Philipson, L. H., Kuznetsov, A., and Nelson, D. J. (1994). Structural
determinant for assembly of mammalian K+ channels. Biophys J 66, 667-673.
Dukes, I. D.,
McIntyre, M. S., Mertz, R. J., Philipson, L. H., Roe, M. W., Spencer, B., and
Worley, J. F., 3rd (1994). Dependence on NADH produced during glycolysis for
beta-cell glucose signaling. J Biol Chem 269, 10979-10982.
Dukes, I. D.,
McIntyre, M. S., Mertz, R. J., Philipson, L. H., Roe, M. W., Spencer, B., and
Worley, J. F., III (1994). Dependence on NADH produced during glycolysis for
ß-cell glucose signaling. J Biol Chem 269, 10979-10982.
Philipson, L. H.,
Malayev, A., Kuznetsov, A., Chang, C., and Nelson, D. J. (1993). Functional and
biochemical characterization of the human potassium channel Kv1.5 with a
transplanted carboxyl-terminal epitope in stable mammalian cell lines. Biochim
Biophys Acta 1153, 111-121.
Philipson, L. H.,
Kusnetsov, A., Larson, T., Zeng, Y., and Westermark, G. (1993). Human, rodent,
and canine pancreatic B-cells express a sodium channel alpha1-subunit related
to a fetal brain isoform. Diabetes 42, 1372-1377.
Philipson, L. H.,
Eddy, R. L., Shows, T. B., and Bell, G. I. (1993). Assignment of human
potassium channel gene KCNA4 (Kv1.4, PCN2) to chromosome-11q13.4 ->q14.1.
Genomics 15, 463-464.
Philipson, L. H.,
and Miller, R. J. (1992). A small K+ channel looms large. TiPS 13, 8-11.
Philipson, L., Hice,
R. E., Schaefer, K., LaMendola, J., Bell, G. I., Nelson, D. J., and Steiner, D.
F. (1991). Sequence and functional expression in Xenopus oocytes of a human
insulinoma and islet potassium channel. Proc Natl Acad Sci USA 88, 53-57.
Lee, T. E., Hice, R.
E., Philipson, L. H., and Nelson, D. J. (1991). Heteromultimeric K+ channels
produced by co-expression of two human K+ channel genes. Biophys Soc abstract.
Chandy, K. G.,
Douglas, J., Gutman, G. A., Jan, L., Joho, R., Kaczmarek, L., McKinnon, D.,
North, R. A., Numa, S., Philipson, L., et al. (1991). Simplified gene
nomenclature. Nature 352, 26.
Philipson, L.,
Schaefer, K., LaMendola, J., Bell, G. I., and Steiner, D. F. (1990). Sequence
of a human fetal skeletal muscle potassium channel cDNA related to RCK4.
Nucleic Acids Res 18, 7160.