Philipson Lab Home Page

Louis H. Philipson MD, PhD
Associate Professor, Department of Medicine
University of Chicago
5841 S. Maryland Ave
Chicago, IL 60637

Phone: 773-702-9180
Fax: 773-702-2771

e-mail: l-philipson@uchicago.edu

l-philipson@uchicago.edu
See my Profile (and other useful stuff) on the Endocrinology Home Page

  http://endocrinology.uchicago.edu


Dave Philipson's homepage

Steve Philipson's homepage

First International Norrbyskar Conference July 3-5, 1999

Cell Physiology at the University of Chicago

Research Interests


Roe, M. W., Fiekers, J. F., Philipson, L. H., and Bindokas, V. P. (2006). Visualizing calcium signaling in cells by digitized wide-field and confocal fluorescent microscopy. Methods Mol Biol 319, 37-66.

Jacobson, D. A., Cho, J., Landa, L. R., Tamarina, N. A., Roe, M. W., Buxbaum, J. D., and Philipson, L. H. (2006). The Downstream Regulatory Element Antagonistic Modulator Regulates Islet Prodynorphin Expression. Am J Physiol Endocrinol Metab.

Gimi, B., Leoni, L., Oberholzer, J., Braun, M., Avila, J., Wang, Y., Desai, T., Philipson, L. H., Magin, R. L., and Roman, B. B. (2006). Functional MR microimaging of pancreatic beta-cell activation. Cell Transplant 15, 195-203.

Fridlyand, L. E., and Philipson, L. H. (2006). Cold climate genes and the prevalence of type 2 diabetes mellitus. Med Hypotheses.

Fridlyand, L. E., and Philipson, L. H. (2006). Reactive species and early manifestation of insulin resistance in type 2 diabetes. Diabetes Obes Metab 8, 136-145.

Fridlyand, L. E., and Philipson, L. H. (2006). Oxidative reactive species in cell injury: mechanisms in diabetes mellitus and therapeutic approaches. Ann N Y Acad Sci 1066, 136-151.

Fridlyand, L. E., and Philipson, L. H. (2006). Reactive Species, Cellular Repair and Risk Factors in the Onset of Type 2 Diabetes Mellitus: Review and Hypothesis. Curr Diab Rev 2, in press.

Chong, A. S., Shen, J., Tao, J., Yin, D., Kuznetsov, A., Hara, M., and Philipson, L. H. (2006). Reversal of diabetes in NOD mice without spleen cell-derived beta-cell regeneration Science 311, 1774-1775.

Tamarina, N. A., Kuznetsov, A., Rhodes, C. J., Bindokas, V. P., and Philipson, L. H. (2005). Inositol (1,4,5)-trisphosphate dynamics and intracellular calcium oscillations in pancreatic beta-cells. Diabetes 54, 3073-3081.

Tamarina, N. A., Kuznetsov, A., Fridlyand, L. E., and Philipson, L. H. (2005). Delayed-rectifier (KV2.1) regulation of pancreatic beta-cell calcium responses to glucose: inhibitor specificity and modeling. Am J Physiol Endocrinol Metab 289, E578-585.

Kuznetsov, A., Bindokas, V. P., Marks, J. D., and Philipson, L. H. (2005). FRET-based voltage probes for confocal imaging: membrane potential oscillations throughout pancreatic islets. Am J Physiol Cell Physiol 289, C224-229.

Fridlyand, L. E., Ma, L., and Philipson, L. H. (2005). Adenine nucleotide regulation in pancreatic beta-cells: modeling of ATP/ADP-Ca2+ interactions. Am J Physiol Endocrinol Metab 289, E839-848.

Philipson, L. H., and Roe, M. W. (2004). Imaging Metabolic and Signaling Targets in the Pancreatic B-cell. Curr Med Chem: Immunol, Endo, Metab Agents 4, 333-337.

Ma, L., Bindokas, V. P., Kuznetsov, A., Rhodes, C., Hays, L., Edwardson, J. M., Ueda, K., Steiner, D. F., and Philipson, L. H. (2004). Direct imaging shows that insulin granule exocytosis occurs by complete vesicle fusion. Proc Natl Acad Sci U S A 101, 9266-9271.

Houamed, K., Fu, J., Roe, M. W., and Philipson, L. H. (2004). Electrophysiology of the Pancreatic beta cell, In Diabetes Mellitus: A Fundamental and clinical text, D. LeRoith, S. I. Taylor, and J. O. Olefsky, eds. (Lippincott, Williams and Wilkins).

Fridlyand, L. E., and Philipson, L. H. (2004). Does the glucose-dependent insulin secretion mechanism itself cause oxidative stress in pancreatic beta-cells? Diabetes 53, 1942-1948.

Yaekura, K., Julyan, R., Wicksteed, B. L., Hays, L. B., Alarcon, C., Sommers, S., Poitout, V., Baskin, D. G., Wang, Y., Philipson, L. H., and Rhodes, C. J. (2003). Insulin secretory deficiency and glucose intolerance in Rab3A null mice. J Biol Chem.

Ueda, K., Lipkind, G. M., Zhou, A., Zhu, X., Kuznetsov, A., Philipson, L., Gardner, P., Zhang, C., and Steiner, D. F. (2003). Mutational analysis of predicted interactions between the catalytic and P domains of prohormone convertase 3 (PC3/PC1). Proc Natl Acad Sci U S A 100, 5622-5627.

Tamarina, N. A., Wang, Y., Mariotto, L., Kuznetsov, A., Bond, C., Adelman, J., and Philipson, L. H. (2003). Small-conductance calcium-activated K+ channels are expressed in pancreatic islets and regulate glucose responses. Diabetes 52, 2000-2006.

Fridlyand, L. E., Tamarina, N., and Philipson, L. H. (2003). Modeling of Ca2+ flux in pancreatic beta-cells: role of the plasma membrane and intracellular stores. Am J Physiol Endocrinol Metab 285, E138-154.

Bindokas, V. P., Kuznetsov, A., Sreenan, S., Polonsky, K. S., Roe, M. W., and Philipson, L. H. (2003). Visualizing superoxide production in normal and diabetic rat islets of Langerhans. J Biol Chem 278, 9796-9801.

Qian, F., Huang, P., Ma, L., Kuznetsov, A., Tamarina, N., and Philipson, L. H. (2002). TRP Genes: Candidates for Nonselective Cation Channels and Store- Operated Channels in Insulin-Secreting Cells. Diabetes 51 Suppl 1, S183-S189.

Philipson, L. H. (2002). beta-Agonists and metabolism. J Allergy Clin Immunol 110, S313-317.

Terracciano, C. M., Philipson, K. D., and MacLeod, K. T. (2001). Overexpression of the Na(+)/Ca(2+) exchanger and inhibition of the sarcoplasmic reticulum Ca(2+)-ATPase in ventricular myocytes from transgenic mice. Cardiovasc Res 49, 38-47.

Shih, D. Q., Screenan, S., Munoz, K. N., Philipson, L., Pontoglio, M., Yaniv, M., Polonsky, K. S., and Stoffel, M. (2001). Loss of HNF-1alpha Function in Mice Leads to Abnormal Expression of Genes Involved in Pancreatic Islet Development and Metabolism. Diabetes 50, 2472-2480.

Qian, F., Huang, P., Ma, L., Kuznetsov, A., Tamarina, N., and Philipson, L. H. (2001). TRP Genes: candidates for non-selective cation channels and

store-operated channels in insulin secreting cells. Diabetes in press.

Bindokas, V. P., Lee, J. P., Li, C., Tamarina, N., A., K., Zhou, Y. P., Wang, Y., Ostrega, D., Roe, M. W., Thompson, C. B., et al. (2001). Overexpression of Bcl-xL in Pancreatic Beta cells Alters Mitochondrial Distribution. submitted.

Zhou, Y. P., Pena, J. C., Roe, M. W., Mittal, A., Levisetti, M., Baldwin, A. C., Pugh, W., Ostrega, D., Ahmed, N., Bindokas, V. P., et al. (2000). Overexpression of Bcl-x(L) in beta-cells prevents cell death but impairs mitochondrial signal for insulin secretion. Am J Physiol Endocrinol Metab 278, E340-351.

Wilson, S. M., Toth, P. T., Oh, S. B., Gillard, S. E., Volsen, S., Ren, D., Philipson, L. H., Lee, E. C., Fletcher, C. F., Tessarollo, L., et al. (2000). The status of voltage-dependent calcium channels in alpha 1E knock-out mice. J Neurosci 20, 8566-8571.

Ma, L., Tamarina, N., Wang, Y., Kuznetsov, A., Patel, N., Kending, C., Hering, B. J., and Philipson, L. H. (2000). Baculovirus-mediated gene transfer into pancreatic islet cells. Diabetes 49, 1986-1991.

Ma, L., Tamarina, N., Wang, Y., Kuznetsov, A., Patel, N., Kending, C., Hering, B. J., and Philipson, L. H. (2000). Baculovirus mediated gene transfer into pancreatic islet cells. Diabetes (rapid publication) 49, 1986-1991.

Kuznetsov, A., Wang, Y., Palmer, M. B., and Philipson, L. H. (2000). Video rate confocal imaging of intracellular free Ca2+ and mitochondrial membrane potential In mouse pancreatic islets. Biophys J 78, 2306.

Philipson, L. H. (1999). Beta-cell ion channels: keys to endodermal excitability. Horm Metab Res 31, 455-461.

Kuznetsov, A., Blair, N., Pfohl, J., Dukes, I. D., and Philipson, L. H. (1999). Dominant-Negative Suppression of Kv2.1 Channels. Biophys J 77.

Zhou, Y. P., Teng, D., Dralyuk, F., Ostrega, D., Roe, M. W., Philipson, L., and Polonsky, K. S. (1998). Apoptosis in insulin-secreting cells. Evidence for the role of intracellular Ca2+ stores and arachidonic acid metabolism. J Clin Invest 101, 1623-1632.

Roe, M. W., Worley, J. F., 3rd, Qian, F., Tamarina, N., Mittal, A. A., Dralyuk, F., Blair, N. T., Mertz, R. J., Philipson, L. H., and Dukes, I. D. (1998). Characterization of a Ca2+ release-activated nonselective cation current regulating membrane potential and [Ca2+]i oscillations in transgenically derived beta-cells. J Biol Chem 273, 10402-10410.

Dukes, I. D., Sreenan, S., Roe, M. W., Levisetti, M., Zhou, Y. P., Ostrega, D., Bell, G. I., Pontoglio, M., Yaniv, M., Philipson, L., and Polonsky, K. S. (1998). Defective pancreatic beta-cell glycolytic signaling in hepatocyte nuclear factor-1alpha-deficient mice. J Biol Chem 273, 24457-24464.

Dukes, I. D., Roe, M. W., Worley, J. F., III  , and Philipson, L. H. (1997). Glucose-induced alterations in ß Cell cytoplasmic calcium: coupling of intracellular calcium stores and plasma membrane ion channels. Curr Opin Endocrinol and Metab 4, 262-271.

Roe, M. W., Worley, J. F., 3rd, Tokuyama, Y., Philipson, L. H., Sturis, J., Tang, J., Dukes, I. D., Bell, G. I., and Polonsky, K. S. (1996). NIDDM is associated with loss of pancreatic beta-cell L-type Ca2+ channel activity. Am J Physiol 270, E133-140.

Roe, M. W., Worley, J. F., III , Mittal, A. A., Kuznetsov, A., DasGupta, S., Mertz, R. J., Witherspoon, S. M., III, Blair, N., Lancaster, M. E., McIntyre, M. S., et al. (1996). Expression and function of pancreatic ß-cell delayed rectifier K+ channels: role in stimulus-secretion coupling. J Biol Chem 271, 32241-32246.

Philipson, L. H., Roe, M. W., Worley III, J. F., Mittal, A., Kuznetsov, A., Blair, N. T., Ghai, C., McIntyre, M. S., Lancaster, M. E., and Dukes, I. D. (1996). Absence Of Glucose-Regulated K(ATP) Current In Pancreatic ß- Cells Of Persistent Hyperinsulinemia. Biophys J 69, abstract.

Marubio, L. M., Roenfeld, M., Dasgupta, S., Miller, R. J., and Philipson, L. H. (1996). Isoform expression of the voltage-dependent calcium channel alpha 1E. Receptors Channels 4, 243-251.

Lee, T. E., Philipson, L. H., and Nelson, D. J. (1996). N-type inactivation in the mammalian Shaker K+ channel Kv1.4. Journal of Membrane Biology 151, 225-235.

Dukes, I. D., and Philipson, L. H. (1996). K+ channels: generating excitement in pancreatic beta-cells. Diabetes 45, 845-853.

Philipson, L. H., and Steiner, D. F. (1995). Pas de Deux or more: The sulfonylurea receptor and K+ channels. Science 268, 372-373.

Philipson, L. H., Roe, M. W., Mittal, A. A., Kuznetsov, A., and Dukes, I. D. (1995). Regulation of Ca2+ oscillations in ßTC3 insulinoma cells. Diabetologia 38(S1), A121.

Philipson, L. H., Kuznetsov, A., Toth, P. T., Murphy, J., Szabo, G., Ma, G. H., and Miller, R. J. (1995). Functional expression of an epitope-tagged G-protein coupled K+ channel (GIRK1). J Biol Chem 270, 14604-14610.

Philipson, L. H. (1995). ATP-sensitive K+ channels: paradigm lost, paradigm regained. Science 270, 1159.

Maya, D. J., Foose, J. M., Philipson, L. H., and Tamkun, M. M. (1995). Localization of the Kv1.5 K+ channel protein in explanted cardiac tissue. J Clin Invest 96, 282-292.

Malayev, A. A., Nelson, D. J., and Philipson, L. H. (1995). Mechanism of clofilium block of the human Kv1.5 delayed rectifier potassium channel. Mol Pharm 47, 198-205.

Ma, G. H., Miller, R. J., Kuznetsov, A., and Philipson, L. H. (1995). k-Opioid Receptor Activates an Inwardly Rectifying K+ channel (GIRK1) by a G protein linked mechanism: co-expression in Xenopus oocytes. Mol Pharm 47, 1035-1040.

Kuznetsov, A., Ma, G. H., Mao, J., Ren, D. J., Worley, J. F. I., Lancaster, M. E., Dukes, I. D., and Philipson, L. H. (1995). Dominant-negative suppression of mammalian delayed rectifier K+ channels by co-expression oif a truncated and tagged channel. Biophys J 68, A266.

Yano, H., Philipson, L. H., Kugler, J. L., Tokuyama, Y., Davis, E. M., LeBeau, M. M., Nelson, D. J., Bell, G. I., and Takeda, J. (1994). Alternative Splicing of Human Inwardly rectifying K+ Channel ROMK1 mRNA. Mol Pharm 45, 854-860.

Williams, M. E., Marubio, L. M., Deal, C., Hans, M., Brust, P. F., Philipson, L. H., Miller, R. J., Jophnson, E. C., Harpold, M. M., and Ellis, S. B. (1994). Structure and functional characterization of neuronal a1E calcium channel subtypes. J Biol Chem 269, :22347-22357.

Stoffel, M., Espinosa, R. I., Powell, K. L., Philipson, L. H., LeBeau, M. M., and Bell, G. I. (1994). Human G-protein-coupled inwardly rectifying potassium channel (GIRK1) gene (KCNJ3): localization to chromosome 2 and identification of a simple tandem repeat polymorphism. Genomics 21, 254-256,.

Roe, M. W., Philipson, L. H., Frangakis, C. J., Kuznetsov, A., Mertz, R. J., Lancaster, M. E., Spencer, B., Worley, J. r., and Dukes, I. D. (1994). Defective glucose-dependent endoplasmic reticulum Ca2+ sequestration in diabetic mouse islets of Langerhans. Journal of Biological Chemistry 269, 18279-18282.

Philipson, L. H., Rosenberg, M. P., Kuznetsov, A., Lancaster, M. E., Worley, J. F., III, Roe, M. W., and Dukes, I. D. (1994). Delayed rectifier K+ channel overexpression in transgenic islets and ß-cells associated with impaired glucose responsiveness. J Biol Chem 269, 27787-27790.

Lee, T. E., Philipson, L. H., Kuznetsov, A., and Nelson, D. J. (1994). Structural determinant for assembly of mammalian K+ channels. Biophys J 66, 667-673.

Dukes, I. D., McIntyre, M. S., Mertz, R. J., Philipson, L. H., Roe, M. W., Spencer, B., and Worley, J. F., 3rd (1994). Dependence on NADH produced during glycolysis for beta-cell glucose signaling. J Biol Chem 269, 10979-10982.

Dukes, I. D., McIntyre, M. S., Mertz, R. J., Philipson, L. H., Roe, M. W., Spencer, B., and Worley, J. F., III (1994). Dependence on NADH produced during glycolysis for ß-cell glucose signaling. J Biol Chem 269, 10979-10982.

Philipson, L. H., Malayev, A., Kuznetsov, A., Chang, C., and Nelson, D. J. (1993). Functional and biochemical characterization of the human potassium channel Kv1.5 with a transplanted carboxyl-terminal epitope in stable mammalian cell lines. Biochim Biophys Acta 1153, 111-121.

Philipson, L. H., Kusnetsov, A., Larson, T., Zeng, Y., and Westermark, G. (1993). Human, rodent, and canine pancreatic B-cells express a sodium channel alpha1-subunit related to a fetal brain isoform. Diabetes 42, 1372-1377.

Philipson, L. H., Eddy, R. L., Shows, T. B., and Bell, G. I. (1993). Assignment of human potassium channel gene KCNA4 (Kv1.4, PCN2) to chromosome-11q13.4 ->q14.1. Genomics 15, 463-464.

Philipson, L. H., and Miller, R. J. (1992). A small K+ channel looms large. TiPS 13, 8-11.

Philipson, L., Hice, R. E., Schaefer, K., LaMendola, J., Bell, G. I., Nelson, D. J., and Steiner, D. F. (1991). Sequence and functional expression in Xenopus oocytes of a human insulinoma and islet potassium channel. Proc Natl Acad Sci USA 88, 53-57.

Lee, T. E., Hice, R. E., Philipson, L. H., and Nelson, D. J. (1991). Heteromultimeric K+ channels produced by co-expression of two human K+ channel genes. Biophys Soc abstract.

Chandy, K. G., Douglas, J., Gutman, G. A., Jan, L., Joho, R., Kaczmarek, L., McKinnon, D., North, R. A., Numa, S., Philipson, L., et al. (1991). Simplified gene nomenclature. Nature 352, 26.

Philipson, L., Schaefer, K., LaMendola, J., Bell, G. I., and Steiner, D. F. (1990). Sequence of a human fetal skeletal muscle potassium channel cDNA related to RCK4. Nucleic Acids Res 18, 7160.